S Asha, S Anitha, R Anantharajan
S Asha, S Anitha, R Anantharajan. Antibacterial activity of herbal plant extracts towards the fish pathogens. The Internet Journal of Microbiology. 2007 Volume 4 Number 2.
Fifteen plant extracts were screened against
Shrimp farmers are loosing millions of dollars annually due to the large scale mortality of shrimp due to diseases caused by antibiotic resistance pathogens. Antibiotics resistance of microorganisms is a global concern (Austin, 1993). Antibiotic resistant
Material and Methods
The leaves of freshly harvested plants were washed and chopped with a knife to pieces. About 10g of chopped leaves were immersed in 50ml of acetone and kept in a shaker overnight at 100 rpm speed. The extract was filtered using Whatman no:1 filter paper. The filtrate was concentrated by keeping the extract in the oven at 40°C. 100 mg of concentrated extract was dissolved in 1ml of acetone. Briefly paper discs of 5mm diameter were prepared using Whatman filter paper (No:1) and sterilized. 5 l of plant extracts was impregnated on the discs (500 g/disc) and was dried at room temperature. In the same way control disc was also prepared by using acetone. Plants that showed antibacterial activity against
Results and Discussion
Each value is the mean standard deviation of minimum three observations.
Spray-dried preparation of
The authors are grateful to the authorities of Manonmanium Sundaranar University, Tirunelveli, for providing required facilities.
R.Anantharajan, Lecturer, Centre for Marine Science and Technology, Rajakkamangalam-629 502, TamilNadu, India. firstname.lastname@example.org Phone: (04652) 223671