Feasibility Of Using Orange Juice For Digestion Of Swamp Eel Viscera For Harvesting Infective Stage Larva Of Gnathostoma Spp.
P Kue-A-Pai, V Wiwanitkit
gnathostomiasis, pepsin, pineapple juice
P Kue-A-Pai, V Wiwanitkit. Feasibility Of Using Orange Juice For Digestion Of Swamp Eel Viscera For Harvesting Infective Stage Larva Of Gnathostoma Spp.. The Internet Journal of Tropical Medicine. 2004 Volume 2 Number 2.
Gnathostoma spinigerum third stage larvae antigen is necessary for Western blot analysis in the diagnosis of Gnathostomiasis. Acid pepsin solution is required for digestion of eel's liver( Fluta alba ) to yield the larvae for antigen preparation. Here, he reported the feasibility of using orange juice as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third stage larvae. From the experimental digestion, it was found that we could recover larvae from orange juice. Thus, orange juice might be another choice for recovering Gnathostoma spinigerum third stage larvae.
According to the recent economical crisis in Thailand, an alternative substance that can be used in stead of acid pepsin is of interest. Many natural products are tested. Recently, Prawang
Materials and Methods
A) Preparation of orange jucie
The oranges were purchased from a local market in Bangkok. The crude green orange juice was prepared as local cooking method.
B) Digestion of swamp eel viscera
All eel livers were separated from other viscera and washed with tap water. The part of eel's liver was digested by the prepared orange juice in the same fashion as done in pepsin digestion. Digestion was performed in a water bath as mentioned earlier, Gnathostoma L3 s were identified and counted by stereomicroscopy.
From the experimental digestion, it was found that we could recover larvae from orange juice. The digestion is similar to those performed by pepsin digestion in our laboratory.
Presently, Western blot analysis, which antigen from parasite third stage larvae is required, is used as gold standard 8. For this process, antigen preparation following acid pepsin digestion is required8. However, acid pepsin is expensive and seems not affordable for many laboratory settings in the developing countries including Thailand.
In this study, the freshly prepared green orange juice (crude) and 1.5 % acid pepsin solution could yield third stage larvae. That means the extract can be effectively used for digestion of swamp eel viscera, similar to that of acid pepsin solution. This unique quality of orange makes it an excellent natural meat tenderizer (Thai cuisine name: Som) This study can assure that crude green orange juice can be alternative to acid pepsin for the recovery of third stage larvae of