S Patel, R Onkarappa, K Shobha
bioethanol, biomass, fermentation, lignocellulosics
S Patel, R Onkarappa, K Shobha. Study Of Ethanol Production From Fungal Pretreated Wheat And Rice Straw. The Internet Journal of Microbiology. 2006 Volume 4 Number 1.
Bioconversion offers a cheap and safe method of not only disposing the agricultural residues, but also it has the potential to convert lignocellulosic wastes into usable forms such as reducing sugars that could be used for ethanol production. This paper reports a preliminary study on the microbial pretreatment and fermentation of the agricultural residues like wheat straw, rice straw. A combination of five different fungi viz.
Bioethanol produced from renewable biomass has received considerable attention in current years. Using ethanol as a gasoline fuel additive as well as transportation fuel helps to alleviate global warming and environmental pollution.
In the last decade, most research has tended to focus on developing an economical and ecofriendly ethanol production process. Much emphasis is being given to the production of ethanol from agricultural and forestry residues and other forms of lignocellulosic biomass.(Kadam
Cellulose is the major constituent of organic matter of plant origin. Lignocellulosic materials are most abundant and renewable resources on earth, which makes them attractive for production of ethanol (Zsolt Szengyel 2000).Pretreatment is an important tool for practical cellulose conversion processes. Pretreatment is required to alter the structures of cellulosic biomass to make more accessible to the enzymes that convert the carbohydrate polymers into fermentable sugars (Mosier
There are several ways to increase the digestibility of cellulose before it is exposed to enzyme or microbial conversion: mechanical, physical chemical or biological pretreatment, as well as the combination of these methods (Bollok 1999).
In this work a study is made on the ethanol production from raw materials which have been treated with various combination of the fungal strains obtained after screening.
Materials And Methods
Wheat straw obtained from local fields of Davanagere district
Rice straw from local fields
Each raw material was powdered and sieved into a 1mm seiver. Powder of each raw material was used as carbon source.
Results And Discussion
Total sugar, reducing sugar, nonreducing sugar, organic carbon, Nitrogen, total solids, moisture content of each raw material was determined.
Initial composition of each raw material is given in the table 1.
Autoclaving for sterilization has affected and resulted in increase in sugar content. With fungal treatment still increase in the yield of sugars was observed. Than individual fungal treatment, the combination of two fungi resulted in high yield of sugars. In case of wheat straw the treatment with
Similar effects were observed as that of reducing sugar yield for protein and cellulase activity. Highest FPU of cellulase was observed in
As highest reducing sugar yield was seen in
Results of all the treatments on each raw material is represented in the tables 2,3.
Microbial treatment of wheat straw and rice straw with
Seema J Patel Lecturer Dept. of Biotechnology GM Institute of Technology Davanagere-577006 Karnataka, India Ph. No.9448223566 Fax No.08192233344 Email ID: email@example.com