Molecular Detection Of Virulence Gene From Nosocomial Staphylococcus Infection In Hospital Kuala Lumpur, Malaysian-A Brief Report
S Masri, R Hamat, M Rahman
gene, methicillin -resistant saphylococcus aureus, molecular methods, nosocomial infections, sequencing
S Masri, R Hamat, M Rahman. Molecular Detection Of Virulence Gene From Nosocomial Staphylococcus Infection In Hospital Kuala Lumpur, Malaysian-A Brief Report. The Internet Journal of Microbiology. 2012 Volume 10 Number 2.
Molecular methods have been proven to be cost-effective for the study of nosocomial pathogen especially Methicillin-resistant
Materials and Methods
A total number of 100 nares specimens were collected from the patients admitted to Pediatric Institute, Hospital Kuala Lumpur during 2010. The samples were examined following standard bacteriological procedure to identify Staphylococcuus aureus (Nordin, 2011). Antibiotic susceptibility test was performed to determine Methicillin-resistant
Molecular technique polymerase chain reaction was performed using published primer to determine mecA gene which is responsible for drug resistant character of the organisms (Nordin, 2011)
MRSA isolate was further subjected to staphylococcal protein A gene (
Results and Discussion
Out of 100 nares specimens examined 25% were
Forty six isolates were subjected to polymerase chain reaction (PCR) for the presence of mecA gene, out of which 14 were mecA positive. Of the 13 positive, 1 was from MRSA and 13 were from Coagulase negative Staphylococcus aureus. This result shows that a large number of coagulase negative staphylococcus that was not previously considered to be pathogens are carrier of mece gene and a potential threat to hospital acquired infections.
In the further study of the one MRSA carrier strain, it was characterized through sequencing and it was observed that the strains possesses SCC
The results of the present study indicate that the percentage of MRSA nasal carriage among paediatric patients is low (1%) compared with those reported by other previous study (Noor et.al., 2008), but we believe that this figure does not reflect accurately the situation in Malaysia because the prevalence of MRSA colonization varies with time and geographical regions.
The study found that mecA gene is highly detected among Coagulase negative staphylococcus isolates. This finding is consistent with a study in India which showed 52% mecA positive among isolated from NICU (Singh et.al.,2009)
One MRSA carrier strain was found to possess SCC
In conclusion the MRSA carrier strain can represent the clonal distribution of locally prevalent MRSA clone. Therefore, a study with large number of strains from many regions is needed to confirm the predominant clones circulating in Malaysia.