D Ofusori, A Adelakun, A Ayoka, J Ibeh, A Adeeyo, A Adesanya, K Ajeigbe
croton zambesicus, cyto-architectural, histological effect, liver, swiss albino mice
D Ofusori, A Adelakun, A Ayoka, J Ibeh, A Adeeyo, A Adesanya, K Ajeigbe. Histological Investigation of the Effects of Croton zambesicus on the Liver of Swiss albino mice. The Internet Journal of Gastroenterology. 2007 Volume 7 Number 1.
Histological effects of
The liver (the largest organ in the body) performs a number of functions some of which are plasma protein synthesis, production of bile and detoxification of most substances 8 . It is note worthy that the liver is also a chemically reactant pool of cells that have a high rate of metabolism, sharing substrates and energy from one metabolic system to another, processing and synthesizing multiple substances that are transported to other areas of the body, and performing myriad other metabolic functions 9 . Based on these varied functions it may be liable to injury particularly in situation of toxicity. It would therefore be expedient to investigate the effects of
Recently we have found that ethanolic extract from the leaves of
Materials And Methods
The leaves of
Preparation of extract
The powdered material (100g) was percolated with ethanol. The extract obtained yield (26.27%) was partitioned between dichloromethane and water. The aqueous fraction was concentrated in vacuum at 20°C (yield 3.98%). The fraction was dissolved in normal saline and administered orally at a dose of 5mg/kg and 10mg/kg as the plant extract for a period of five consecutive days.
Fifteen Swiss male albino mice (27-30g) were used for the experiment. They were maintained under standard laboratory conditions in the Animal Holdings of Igbinedion University, Okada, Nigeria, and fed with standard pelleted diet and water
Histological examination was done by fixing the organs of the mice in 10% formol saline, processed and embedded in paraffin wax. Tissue blocks were sectioned 5 µm thick and stained with Haematoxylin and Eosin (H & E).
The treatment and control sections of the liver showed normal histological features with the hepatic lobules showing polyhedral three-dimensional shape. Sinusoids originate at the lobule margin and course between plates of hepatocytes to converge upon the terminal hepatic venule. The lobules were also seen to be bounded by thin septa of collagenous supporting tissue. (Figure2, 3 & 4).
Our histological findings revealed that there was no distortion on the cyto-architecture of the liver parenchyma both in the treated and control groups. This confirms that consumption of
Chemosuppressant potential of
The present investigation justifies that intake of ethanolic extract of
The authors are in deed very grateful for the technical assistance of E. Gbela of the Department of Morbid Anatomy and Forensic Medicine, Obafemi Awolowo University, Ile-Ife, Nigeria.