diabetes mellitus, lectrin, leucocytes
S Koley. The Anti – LH Lectin – Leucocyte Reactions in Patients with Diabetes Mellitus: Further Observations. The Internet Journal of Biological Anthropology. 2007 Volume 1 Number 2.
Leucocyte samples from 109 confirmed cases of diabetes mellitus were tested with the anti-LH lectin,
Human leucocytes possess A and B antigens 1,2,3,4,5. By absorption and elusion tests and also by mixed cell agglutination test, the agglutination results have been confirmed. According to Dausset 6, the A and B antigens are intrinsic in leucocytes and not merely absorbed from the plasma. Lectin – leucocyte interactions have been studied by Sharon 7, Sehrt and Luther 8, Culina et al 9 and Carlin et al 10.
The anti-LH lectin (
Materials And Methods
A total of 109 blood samples from patients with diabetes mellitus (type II) were obtained from District Hospital, Sagar, and Varny Pathology Clinic, Sagar, Madhya Pradesh. Control samples were obtained from 98 unrelated normal healthy individuals from the same area. The samples comprised both males and females.
For the ABO typing, standard serological procedures were followed. The anti-LH lectin (
The separation of leucocytes was done following Widmann 15, with minor modifications. For leucocyte agglutination tests, 25 µl leucocyte suspensions and an equal amount of anti-LH lectin were mixed in a cavity of a tile. Saline control was also made for comparison. After 20 minutes, readings were recorded both macroscopically and microscopically. Thereafter, titration tests were performed and results were recorded after 30 minutes.
Table 1 shows the anti-LH lectin –leucocyte reactions in diabetic and control samples at different titres. The lectin
The distribution of titre scores in patients with diabetes mellitus and controls pooling all the four blood groups is given in table 2. The lectin reacted at titre 1:16 slightly more with diabetic cells (30.27% and) than controls (29.59%) and at titre 1:32 slightly less (69.72%) than controls (70.41%), showing no significant differences (P> 0.05) statistically between the diabetic and control samples.
The results indicateed that the lectin