V Rajamanickam, A Rajasekaran, S Darlin quine, M Jesupillai, R Sabitha
couroupita guianensis, plant extract and nematode
V Rajamanickam, A Rajasekaran, S Darlin quine, M Jesupillai, R Sabitha. Anthelmintic activity of the flower extract of Couroupita guianensis. The Internet Journal of Alternative Medicine. 2008 Volume 8 Number 1.
The chloroform, acetone and ethanolic flower extracts of
Materials And Methods
The flowers were collected from western ghates of Virudunagar District, Tamilnadu, India and authenticated by Dr. Stephen, Dept. of Botany, The American College, Madurai, Tamilnadu, India. A voucher specimen (CG) have been kept in our laboratory for future reference.
Preparation of the extract
The coarsely powdered flower materials were extracted exhaustively with chloroform, acetone and ethanol using Soxhlet apparatus(6). These extracts were concentrated under reduced pressure and preserved in desiccators until further use.
The method described by Dash et al(7-8) was employed for evaluating anthelmintic activity.
Fifty milliliter of test solution containing 20, 50 and 100 mg /ml of test extracts (Chloroform, acetone and ethanol extracts of flowers of
The Mean time of paralysis and death was recorded in minutes. The paralysis time was recorded when no movement of any sort could be observed except when the worms were shaken vigorously. Time for death of worms were recorded when worms were neither moved while shaken vigorously nor when dipped in warm water (500C).
All the values were represented as Mean ± S.E.M using student ‘‘t’‘ test. P< 0.001 (Compared to standard) was considered significant.
Result And Discussion
Chloroform, acetone and ethanol extracts of flowers of CG showed significant anthelmintic activity (table.1) at the concentration 50 mg/ml and 100mg/ml against Pheritima poshthuma. Activity was found to be increased with dose (shortest time of paralysis and death was observed at 100 mg/ml) and the activity was comparable to the well known anthelmintic agent Piperazine citrate.
Student ‘‘t’‘ test, *P< 0.001 (Compared to standard) was considered significant.
Indian earth worms resembles intestinal round worm parasite of human beings(9-11).Phenolic compounds by uncoupling oxidative phosphorylation hinder the energy production in helminth parasites(12). Phytochemical analysis of flowers of CG showed the presence of phenolic substances. Therefore, we conclude that phenolic compounds may be responsible for the anthelmintic activity of flowers of CG. Further study is in progress to isolate active constituent responsible for the activity.