Polyphenol analysis and Antitumor activity of Crude extracts from Tegmen of Artocarpus heterophyllus.
N Rajendran, J Ramakrishnan
antitumor and hep2 cells, artocarpus heterophyllus, polyphenol, tegmen
N Rajendran, J Ramakrishnan. Polyphenol analysis and Antitumor activity of Crude extracts from Tegmen of Artocarpus heterophyllus.. The Internet Journal of Alternative Medicine. 2008 Volume 7 Number 2.
Many medicinal and food plants contain large amounts of antioxidants other than Vitamin C, Vitamin E and Carotenoids. The antioxidative effects are mainly due to Phenolic acids, flavonoids, and phenolic diterpenes. Natural products are reportedly beneficial to physiological health. Various favonoids and non-favonoids have been reported as showing radical scavenging activity (Sawa
Moracea is large family comprising sixty genera and nearly 1400 species, including important group such as
Materials And Methods
Preparation of plant extract
About 25 g of the inner thin membranous brown tegmen of the Jack fruit seed was and taken in a conical flask and immersed in 100 ml of organic solvent namely ethanol, Methanol and Butanol. It was incubated at room temperature at different time intervals like 2, 12 and 24 hr at 150 rpm. After incubation time, the suspension was filtered and the solvent was evaporated. The extract was concentrated to dryness and dissolved in 0.25% Dimethyl Sulphoxide (DMSO, Merck) to the concentration of 100 mg / ml.
Determination of total polyphenol contents
Total polyphenol contents in the extracted powder from the tegmen were determined by the Folin-Ciocalteu colorimetric method (Ough and Amerine, 1988 and Kumazawa
The antitumor assay was performed on human laryngeal epithelioma (HEp2) cells obtained from King Institute of Preventive Medicine, Chennai, India. The cells were grown in 24 well plate (Falcon) in Eagle’s Minimum Essential Medium (Hi Media) supplemented with 10% fetal bovine serum (Gibco Laboratories) and 1% antibiotics (streptomycin, penicillin-G, kanamycin, amphotericin B, Hi Media). The cell suspension (105 cells / ml) was seeded in every well and incubated at 37oC for 48 hr in 5% CO2 for the formation of confluent monolayer. The monolayer of cells in 24 well plates was exposed to various dilutions of the methanolic extract. The cell viability was measured using MTT assay as described by Mosmann (1983) using MTT (5 mg / ml) and DMSO. Cell control was maintained throughout the experiment and the assay was performed in triplicates.
The extractive yield of extracts from the tegmen of
The tumor cell suppression potential of methanolic extract
The extractive yield of sample was higher in methanol, compared to ethanol and butanol. The yield obtained decreased with decrease in polarity. Since methanol has high polarity, it could dissolve both the polar and non polar compounds in it.
The methanol extracts of sample inhibited nearly 100 % of HEp2 cells up to 1:4 dilution of the crude extract and started decreasing with increase in dilution. Arpornsuwan and Punjanon (2006) reported that the methanolic extract of
The extractive value, total polyphenolic content and antitumor activity was at its peak in methanolic extract indicating that most of the active components are extracted with methanol. Cytotoxic changes observed was cell aggregation, cell rounding and cell death. The overall results indicates the promising baseline information for the potential uses of the methanol extracts of tegmen of
We thank SASTRA University for providing us the facilities and requisite support for completion of this work. We express our thanks to Life Teck Research Centre, Chennai for analysis of samples for antitumor activity.