A Kumar, K Lakshman, K Jayaveera, K Satish, S Tripathi
hptlc, quercetin, rutin, standardization, terminalia chebula
A Kumar, K Lakshman, K Jayaveera, K Satish, S Tripathi. Estimation of rutin and quercetin Terminalia chebula by HPLC. The Internet Journal of Aesthetic and Antiaging Medicine. 2008 Volume 2 Number 1.
Tannins and Flavonoids present in the
Materials and Methods
The Shimadzu class LC-10AT HPLC, Hichrom C18 and a Rheodyne 7725i injector fitted with a 20 l loop, column oven, and a photodiode array detector. The output signal was monitored and processed using chromquest version3.0 software on Pentium computer (Hewlett Packard).
Solvent and Chemicals
Rutin and quercetin obtained from natural remedies (Bangalore) chromatographic grade methanol, formic acid and acetonitrile (AR), were obtained from Merck (Mumbai, India).
Preparation of Standard and Sample Solutions
Rutin and quercetin 10 mg were accurately weighed into a 10 mL volumetric flask, dissolved in 5 mL methanol and the solution was made up to 10 mL with the same solvent (1 mg/mL).
Results And Discussion
The retention time of standards rutin and quercetin were found to be 4.072 and 19.104 (Graphs1). The retention time of rutin and quercetin in
The authors are thankful to K.V.Naveen Kiran, Chairman, Sri K.V.College of Pharmacy, Chickballapur, for providing required facilities.