Influence of Carica Papaya Linn Extracts on Paracetamol and Thioacetamide Induced Hepatic Damage in Rats
: carica papaya extracts cpe, and thioacetamide tta, paracetamol pcm
S Kantham. Influence of Carica Papaya Linn Extracts on Paracetamol and Thioacetamide Induced Hepatic Damage in Rats. The Internet Journal of Pharmacology. 2009 Volume 9 Number 1.
The present investigation was designed to evaluate the effects of
Materials And Methods
Preparation of C. papaya Plant Extract
The fruits of C. papaya were brought from a local market in Warangal city, Andhra Pradesh, India. The ripen fruits of
To prepare ethanol extract 200 g of powdered fruits of papaya were suspended in 2 liters of petroleum ether and kept in refrigerator overnight for removing all the fatty substances. The supernatant was discarded by filtration with fine cloth and the residue was kept open to dry out the petroleum ether by air. Then the residue material was mixed with 2 liters of 90% ethanol for 48 hours and being filtered, kept for another 24 hours with ethanol followed by re-filtration by vacuum pump. Then the whole extract was concentrated with rotary vacuum evaporator. Then the extract was put into freeze dryer to make it powder. Finally the extract was stored in refrigerator.
Adult Wistar strain albino rats (weighing, 150-200 g) were used for the study. Animals were supplied by Animal House Facility of Guru Nanak Institute of Pharmacy, Ibrahimpatnam, Hyderabad, A.P., India (Ref. No: 1374/ac/10/CPCSEA) and kept under standard laboratory conditions in polypropylene cages in 12-h light/dark cycle at 25 ± 2°C. Animals were provided with standard pellet diet (Lipton, India) and water
Paracetamol (PCM) induced hepatic damage
The animals were divided into six groups (n=6) and treated for 10 days orally. The animals of Group I and II were treated with vehicle for 10 days. Group III animals received silymarin (100 mg/kg,
Thioacetamide (TAA) induced hepatic toxicity
The animal groupings and treatment was similar to that used in PCM induced liver damage. Single dose of TAA (100 mg/kg,
Paracetamol induced liver toxicity
After 48 hr of administration of PCM, the serum levels of ALT, AST, ALP and bilirubin were markedly increased when compared to normal control. Pretreatment with low dose of
Thioacetamide induced liver injury
The effects observed in this model were almost similar to that observed in PCM induced hepatotoxicity. The medium (250 mg/kg,
The present study reveals the hepatoprotective activity of
The results of the present study supports the traditional claim that fruits of
The authors are thankful to the Director and Principal, SRL Institute of Pharmaceutical Sciences, Kakatiya University, Kummarigudem Road, Madikonda, Kazipet, Warangal, Andhra Pradesh, India, and Department of Pharmacology, Guru Nanak Institute of Pharmacy, Ibrahimpatnam, Hyderabad, A.P., India for providing laboratory facilities and financial support.