agar well diffusion., antimicrobial activity, nerium indicum, thevetia peruviana
B REDDY. Antimicrobial Activity Of Thevetia Peruviana (Pers.) K. Schum. And Nerium Indicum Linn.. The Internet Journal of Pharmacology. 2009 Volume 8 Number 2.
The main objective of the present study was to analyze the antimicrobial potential of various concentrations (2mg, 4mg, 6mg and 8mg/well) of leaf extracts of
Medicinal plants use is widespread. The production of medicines and the pharmacological treatment of diseases began with the use of herbs. Plants historically have served as models in drug development for some major reasons. The first being that each plant is a unique chemical factory capable of synthesizing large number of highly complex and unusual chemical substances. The second reason involves biologically active substances derived from plants have screened as templates for synthesis of pharmaceuticals. While the reason concerns the fact that highly active secondary plant constituents have been instrumental as pharmacological tools to evaluate physiological processes. One of the paramount reasons for pursuing natural products chemistry resides in the actual or potential pharmacological activity to be found in alkaloids, terpenoids, coumarins, flavonoids, lignans etc.
Due to the diverse medicinal properties attributed to the plants, the present investigation was undertaken to analyze the antimicrobial potential of two locally available wild plants namely,
Materials And Methods
Collection of Plant materials: The leaves of
Preparation of the Extracts: The 100g leaves of
Microorganisms Used: Clinical laboratory bacterial isolates of
Culture Media: The nutrient agar, nutrient broth and Sabouraud dextrose agar were purchased from HiMedia, Laboratories Limited, Mumbai. Nystatin an antifungal agent, purchased from, Tocelo chemicals, Netherlands, Streptomycin (powdered form) another antibiotic from Nanjing Asian chemicals Co., Ltd. The solvents and other chemicals used were analytical grade.
Antimicrobial activity: Here the
Statistical Analysis: All the data are expressed as mean ± S.E.M. (standard error of the mean). The significance level was determined using the Student‘t’ test. A
It is evident from the results that, the extracts of both the plants significantly inhibited the growth of all the tested pathogens in a dose dependent manner. The test organisms like
The results imply that, the extracts of both the plants exhibited more or less pronounced antibacterial and antifungal potencies affecting Gram positive, Gram negative bacteria and fungi used in the present study. Several earlier reports also supporting the present research study. The chloroform and methanolic extracts of
In conclusion, the alcoholic extracts of