S Salil, A Bhupinder, A Basheer
blood., inula racemosa, lipid peroxidation, liver, reduced glutathione
S Salil, A Bhupinder, A Basheer. Antioxidant Properties Of Inula Racemosa, A Traditional Herbal Medicine. The Internet Journal of Pharmacology. 2012 Volume 10 Number 1.
The noxious consequences of oxidative stress induced by reactive oxygen species in biological systems are manifold and include oxidative damage to vital biomolecules, alterations in some physiological processes and several ailments including diabetes, cardiovascular diseases, respiratory disorders and cancer (1,2). Peroxidation of membrane lipids, has been suggested to be one of the primary mechanism of cell injury associated with various diseases and chemical toxicities (2). Reduced glutathione plays an important antioxidant role in the maintenance of sulfhydryl dependant enzymes activity and integrity of biological membranes during intracellular redox potential imbalance occurring in physiological and pathological conditions (3). These observations have prompted extensive studies towards the development of therapeutic antioxidants and elucidation of their mechanism of action.
According to a WHO estimate ,80% of the people in developing countries of the world rely on traditional medicine for their primary health care needs, and about 85% of traditional medicines involve the use of plant extracts (4). However, much less information is available regarding their active principles, detailed pharmacology and toxicology profile and mechanism of action. The root of
Material and Methods
Root of inula racemosa were purchased from local market, dried in shade and grinded with the help of electric grinder to a fine powder. Root powder was extracted with 70% ethanol in soxhlet apparatus at 55-60ᵒ C. The alcoholic crude extract was filtered and concentrated on a waterbath under reduced pressure to obtain a thick viscous residue/resin which was further dried in a vaccum desiccator over anhydrous calcium chloride. The yield of residue thus obtained was 155 gm/kg of the dried roots.
Albino rats of either sex weighing 100-150g were maintained under standard laboratory conditions. Animals were divided into two groups of five rats each. Experimental animals received the herb resin (in 1% gum acacia) orally with the help of a feeding cannula at a dose of 60mg resin/ kg for 21 consecutive days. Controls were administered only gum acacia and were run under similar experimental conditions.
Animals were killed 24 hours after the last dose of plant extract and samples of blood were collected in EDTA (1mg /ml blood) for biochemical estimations. Livers were immediately removed, washed with saline and homogenized in cold buffered-KCL solution(1.15%KCL in 0.01M Tris –Hcl buffer, pH 7.4) with help of Potter-Elvehjem homogenizer.
Lipid peroxidation was measured in whole blood in terms of thiobarbituric acid reacting species (TBARS) (7) and GSH was determined in whole blood and liver homogenate using 5,5’-dithiobis-2-nitrobenzoic acid (DTNB) as described by Jollow et al (8).Statistical Analysis- Mean ± S.E. was calculated for control and treated samples separately and significance was determined by students “t’ test.
Results and Discussion
The above table (Table no. 1) shows that oral administration of alcoholic extract of