S Salam, A Khan, M Mir
amoebotaenia sphenoides, free range chicken, kashmir valley
S Salam, A Khan, M Mir. Prevalence and Pathology of Amoebotaenia sphenoides in free ranging chicken of Kashmir Valley.. The Internet Journal of Parasitic Diseases. 2008 Volume 4 Number 1.
In the present study, a survey was carried out for a period of two years from Jan 2005 to Dec 2006 on the prevalence and pathology associated with the cestode
Materials And Methods
In the present study free ranging chicken were purchased from villagers and the local market from different localities covering almost whole of the Kashmir valley. Post mortem examinations were performed as described by Fowler (1990). After decapitation, the trachea was examined directly and the entire gastrointestinal tract including the esophagus was collected from each bird. The gastrointestinal tract was opened in a longitudinal section and the contents were carefully washed through a test sieve. The mucosa was scraped in order to collect the cestodes embedded in the mucosal layer. Finally, the contents were also examined under the microscope for locating any larvae or egg invisible to naked eye. All cestodes were counted before being fixed. Permanent slides of the scolices were prepared to identify the cestodes taking help from the work of Yamagutti and Soulsby (1982). The intestines showing gross abnormalities were fixed in 2.5% glutaraldehyde for Scanning Electron Microscopic studies and for histological studies the infected intestinal tissues were fixed and preserved in 10% formalin, processed through conventional technique (Behmer et al., 1976) for paraffin embedding and then sectioned at 4-5 microns in thickness. The sections were then stained with Haematoxyline and Eosin.
The monthwise prevalence of
In the fowl intestines infected with
The present investigation on the prevalence of the cestode
Histological studies of the infected tissues revealed atrophy of villi with broadened surface and increased vascularity. The epithelium and glands at the site of infection were disintegrated and degenerated. The inflammatory reaction was characterized by predominant infiltration of heterophils and lymphocytes and proliferation of connective tissues suggestive of mild form of enteritis. Infiltration of heterophils and lymphocytes probably acuted as first and second line of defense (Runnels