A Doss, A Pichai Anthony Doss, R Dhanabalan
A Doss, A Pichai Anthony Doss, R Dhanabalan. In vitro Antioxidant Properties of Certain Indigenous Medicinal Plants From Western Ghats of India. The Internet Journal of Nutrition and Wellness. 2008 Volume 7 Number 1.
Antioxidant activity (DPPH & reducing power assay),
Nature still serves as the man's primary source for the cure of his ailments. The majority of the rich diversity of Indian medicinal plants is yet to be scientifically evaluated for such properties. However, the potential of higher plants as source for new drugs is still largely explored (Oke
Materials and Methods
Plant samples of the selected species viz.
Preparation of extracts
25 g of the dried powdered samples from
The antioxidant activity of Plant extracts were determined by different in-vitro methods such as, the DPPH free radical scavenging assay and reducing power methods. The different extracts were dissolved in methanol at the concentration of 2mg/ml. all the assays were carried out in triplicate and average value was considered.
DPPH Radical scavenging activity:
DPPH scavenging activity was carried out by the method of Blois, (1957).Different concentrations (1000,500,250,125,62.5 and 31.2 mg/ml) of
IC 50 Value was also calculated.
Reducing activity was carried out by using the method of
Results and Discussion
Scavenging activity for free radicals of 1,1-diphenyl 1-2-picryl hydrazyl (DPPH) has been widely used to evaluate the antioxidant activity of natural products from plant and microbial sources. Plant extracts from 3 medicinal plants listed in table.1.were prepared for investigation of their antioxidant activities. Free radical scavenging activity of methanolic extract from the 3 medicinal plants was quantitatively determined using DPPH and reducing power assay. Table 1-3 shows the result of antioxidant assay.
All the medicinal plant materials used contain varying degrees of antioxidants. It is well recognized that free radicals are critically involved in various pathological conditions such as cancer, cardiovascular disorders, arthritis, inflammation and liver diseases (Martin
Values are expressed as mean ±S.D
Values are taken as a mean of three individual experiments
Against the backdrop of many known medicinal properties of these plants, results from the present work suggest that relatively low values of antioxidant and reducing power may not imply a low medicinal value. Emerging trends in antioxidant research point to the fact that low levels of phenolics (and other phytochemicals) and low value of antioxidant indices in plants do not translate to poor medicinal properties. The radical scavenging effects of
The authors express their sincere gratitude to the management of RVS College of Arts and Science, Coimbatore.
A.Doss Department of Microbiology RVS College of Arts and Science Coimbatore, Tamilnadu, India email@example.com