I Omalu, V Ayanwale, A Ajalaruru, A Mohammed, J Bala, V Chukwuemeka
maggot, microbes, microorganisms
I Omalu, V Ayanwale, A Ajalaruru, A Mohammed, J Bala, V Chukwuemeka. Isolation Of Fungi And Bacteria From Housefly (Musca Domestica L.) Larvae. The Internet Journal of Microbiology. 2009 Volume 9 Number 1.
Housefly larvae were cultured on meat and collected for the isolation and identification of microorganisms associated with them. The microbes were cultured from both the gut and body surface of the maggot on nutrient agar (for bacteria) and potato dextrose agar (for fungi) and incubated at about 37°C for 48 hrs before observations. A variety of microorganisms, which includes the pathogenic
They are scavengers, consuming large quantities of decaying animal matter. However, some are injurious to humans by virtue of the fact that they feed on and breed in trash, decaying flesh, and bodily waste. This behaviour facilitates the spread of diseases such as tuberculosis, typhoid fever, cholera, diarrhoea, Staphylococcal food poisoning and Shigellosis ,
. Housefly larvae, or maggots, will eat most decaying animal or vegetable matter . The housefly (
Materials and Methods
Fresh meat was exposed in shade for the infestation of houseflies. The developed maggots were collected with sterile forceps. Aseptic procedures were carried out so as to minimize contamination from bacteria not associated with the collected maggots.
The following media were used in culturing the microorganisms; nutrient agar and potato dextrose agar.
Isolation of Internal and External Microbes
Two maggots were allowed to move freely on the solidified agar media for 5 min so that they can deposit the microbes on them on the agar media. Two maggots were placed in a beaker containing 10 ml sterile water and thoroughly mixed together by shaking the beaker in order to ensure even distribution of the particles on the maggots. 0.5 ml of the suspension was pipette into molten agar media. The preparation was gently mixed together. For the external microbes, maggots were first surface sterilized by placing them in 70% ethanol and then rinsing in sterile water. A sterile blade was then used to dissect the maggots thereby revealing the gut. A maculating loop was flamed and allowed to cool and then it was used to obtain exudates of the gut. The obtained exudates were streaked on the solidified agar media .
The nutrient agar and potato dextrose agar were used to culture both external and internal microbes. Then 0.5 ml of the body surface suspension was pipetted and transferred into prepared nutrient agar and potato dextrose agar in a Petri dish. To culture the microbes from the gut, 0.5 ml solution of already dissected 2 maggots in 10 ml in distilled water was pipetted and poured into the already prepared potato dextrose agar and nutrient agar. The test tubes are then plugged with non-absorbent cotton swab, and then properly sealed with aluminium foil. The Petri dishes placed in an incubator under a temperature of 37°C for 48 h.
After incubation period, the Petri dishes were checked for microbial growth. In order to identify the microorganisms cultured on the agar, they were sub-cultured on already prepared nutrient agar and potato dextrose agar. The Petri-dishes containing the media were placed in an incubator under a temperature of about 37°C for 48 h (for bacteria) and 4-5 days (for fungi). After the incubation period, the isolates were observed and identified.
Characterization and Identification of Microbial Isolates
Bacterial isolates were characterised and identified using the following, gram staining, catalase, coagulase, motility and starch hydrolysis tests. The fungi isolates were characterized based on the colour of aerial and substrate hyphen. In addition, the nature and shape as well as the presence of special structures e.g. rhizoid were noted other structures were examined.
It was observed that the bacterial isolates recovered were
The houseflies are much more than a nuisance and that they pose serious health risks as mechanical vectors.
It was observed that the bacteria isolates recovered were gram positive bacteria
Houseflies move from animal or human faeces to food that will be eaten uncooked by humans. Also, when consumed by flies, some pathogens can be harboured in the mouthparts or alimentary canal for several days, and then be transmitted when flies defecate or regurgitate. In situations where plumbing is lacking, such as open latrines, serious health problems can develop, especially if there are outdoor food markets, hospitals, or slaughter houses nearby. Among the pathogens commonly transmitted by houseflies are