P Prema, S Audline Suja, S Ranjani, G Immanuel
anti-candidial activity, s. albidoflavus, s. aureofaciens, uv and ftir.
P Prema, S Audline Suja, S Ranjani, G Immanuel. UV/VIS, FTIR spectrum and Anticandidial activity of Streptomyces strains. The Internet Journal of Microbiology. 2008 Volume 7 Number 2.
Two different species
Materials and Methods
Collection and maintenance of Actinomycetes strains
antagonism (Slavica et al., 2005)
Balanced sensitivity medium (BSM, Difco) plates were prepared and inoculated with individual
Isolation of anticandidial compound
For the detection of anticandidial activity of
Ultraviolet (UV) and Fourier transform infrared (FTIR) spectral analysis
The absorption spectrum of each active extract was determined in the UV region (200-400nm) by using a Shimadzu -1601 UV-VIS spectrophotometer (Sahin and Ugur, 2003). The spectra were recorded at 200-400 nm range. The FTIR spectrum of each active extract was detected by using Shimadzu IR-470 (Augustine et al., 2005). The spectra were scanned in the 400 to 4000 cm-1 range. The spectra were plotted as intensity versus wave number.
Results and Discussion
Spectral analysis of the anticandidial compound
Figs. 3 and 4 show the FTIR spectrum of the anticandidial compound from
The need for new, safe and more effective antifungals is a major challenge to the pharmaceutical industry today, especially with the increase in opportunistic infections in the immuno compromised host. The biotechnological potential of