R Srivastava, Shalini
antifungal, inhibition, microorganism, plant pathogens, pseudomonas, resistant
R Srivastava, Shalini. Antifungal Activity of Pseudomonas fluorescens Against Different Plant Pathogenic Fungi. The Internet Journal of Microbiology. 2008 Volume 7 Number 2.
Antifungal activity of different strains of
The surfaces of aerial plant parts provide a habitat for epiphytic micro-organisms, many of which also influence the growth of pathogens. Bacteria are generally the predominant initial inhabitants of newly expanded leaves, while yeasts and filamentous fungi dominate later in the growing season 1. A large body of information has been accumulated regarding antagonism between bacteria and fungi on the leaf surface, and its possible role in the biological control of pathogenic fungi 2. Biological control may be an alternative to chemicals in the control of some pathogenic fungi, or in order to reduce environmental pollution. Saprophytic organisms play an important part in reducing the incidence of foliar diseases from fungi and bacteria on crops in the field 3, 4.
Antagonistic activity was also observed for
The purpose of this study was to examine the antifungal activity of
Materials and Methods
Isolation of cultures
Five strains of
Test organisms and culture media
Test fungi were isolated on potato dextrose agar (PDA) (peeled potato 250 g, dextrose 20 g, agar 15 g, distilled water 1 L) medium from their respective hosts collected from experimental farm of Banaras Hindu University, Varanasi, India. The cultures were further purified by single spore isolation technique and maintained at 25±2OC on PDA slants. 7-10 days old culture were used in the experiment. The fungi included in the present study are
Results and Discussion
Five strains of
All the five strains showed varied levels of antifungal activity and the highest concentration of
The biological control of soil-borne pathogens with antagonistic bacteria, particularly
Recent increases in fungal infections, the few available antifungal drugs, and increasing fungal resistance to the available antifungal drugs have resulted in a broadening of the search for new antifungal agents. Strains of
The presented data exhibit the antifungal activity of
We are grateful to the Institute of Bioengineering and Biological Sciences, Varanasi, India to provide financial support and lab facilities to carry out the present investigation.