B Uma, K Prabhakar, S Rajendran, Y Lakshmi Sarayu
albizzia lebbeck. benth, antimicrobial activity, candida albicans., diarrhoeal pathogens, salmonella typhimurium, shigella dysenteria
B Uma, K Prabhakar, S Rajendran, Y Lakshmi Sarayu. Antimicrobial Activity of Albizzia Lebbeck Benth against Infectious Diarrhoea. The Internet Journal of Microbiology. 2008 Volume 7 Number 1.
Diarrhoea is a major public health problem in developing countries and is said to be endemic in many regions of Asia especially in India, and is the leading cause of high degree of morbidity and mortality among paediatrics. Enteric bacteria such as
Materials And Methods
The bark of
Preparation of Extracts
The barks were shade dried and powdered. The powdered bark was extracted with various solvents, aqueous, methanol, chloroform, petroleum ether and hexane using maceration techniques. Twenty-five grams of powdered bark was extracted with 125 ml of solvent with occasional shaking for 3 days at room temperature. The extracts were filtered, concentrated and dried at 50oC and the weight of each residue was recorded and percentage yield was calculated.
Isolation of Enteric pathogens
One hundred paediatric patients with complaints of diarrhoea, attending Rajah Muthiah Medical College, Chidambaram, during June’ 2008 was screened for enteric pathogens. The specimens were examined by Gram’s stain and were inoculated in Sabouraud’s dextrose agar with chloramphenicol for fungi and Blood agar, MacConkey agar for bacterial pathogens. The confirmation of enteric pathogens was done based on standard techniques (Collee
Screening of Antimicrobial activity
The antimicrobial screening was evaluated against Enterotoxigenic
Determination of Minimum Inhibitory Concentration
The extracts that showed antimicrobial activity by disc diffusion method were subjected to minimum inhibitory concentration (MIC) assay by using two-fold serial dilution method. MIC’s were interpreted as the lowest concentration of the sample, which showed clear fluid without development of turbidity. All the MIC tubes, which did not show any turbidity, were streaked over the Muller–Hinton agar plates for bacteria and Sabouraud’s dextrose agar plates for
The methanol extract of
Results and Discussion
Total of one hundred diarrhoeal paediatric patients was screened and 52 (91.2%) bacterial pathogens and 5 (8.8%)
Above values are the means of three assays. *indicates number of isolates; - indicates no activity; nt – not tested, 1+ indicates zone of inhibition in average of 7 to 10mm; 2+ indicates zone of inhibition in average of 11 to 14mm; 3+ indicates zone of inhibition in average of 15 to 18mm; 4+ indicates zone of inhibition in average of 19 to 22mm. ETEC- Enterotoxigenic
The results reveal that the methanol extract of
In rural India, 70 percent of the population is dependent on the traditional system of medicine. To determine the potential and to promte the use of herbal medcine, it is essential to intensify the study of medicinal plants.