R Sharada, S Ruban, M Thiyageeswaran
antibiogram, e. coli, pathological conditions, poultry, serotypes
R Sharada, S Ruban, M Thiyageeswaran. Antibiotic Resistance Pattern Of Escherichia Coli Isolated From Poultry In Bangalore. The Internet Journal of Microbiology. 2008 Volume 7 Number 1.
A total of 85 samples were collected from moribund birds with different pathological conditions like perihepatitis, enteritis, air sacculitis and pneumonia. In this investigation thirty two avian pathogenic
Concern has been expressed about possible harmful effects on humans through the use of drugs in agriculture, as follows: 1) increased microbial drug resistance, 2) drug residues in food, 3) allergic reactions and sensitisation to antimicrobials, and 4) drug toxicity (Bazile-Pham-Khac
Materials and Methods
Collection of Samples: The tissues were collected based on clinical findings and pathogonomic lesions observed during detailed post mortem examination of poultry at Department of Pathology, Veterinary College, Bangalore; Poultry Disease Diagnostic Laboratory, Bangalore and Central Disease Investigation unit, IAH & VB, Bangalore, India. A total of 85 samples from 55 birds of 1-7 weeks of age were collected in sterile containers following aseptic precautions and transported to laboratory. Tissues were collected form cases exhibiting perihepatitis (31), enteritis (27), airsacculitis (5), yolk sac infection (7), pnemonitis (1) and pericarditis (14).
Isolation and Identification: The tissue samples were plated on Mac Conkey agar (HIMEDIA) and incubated at 37o C for 24 hours. The lactose fermenting colonies were reinoculated to Eosin Methylene Blue (HIMEDIA) agar and colonies producing metallic sheen were transferred to Nutrient agar slants and incubated at 37o C for 24 hours and stored at 4o C for further identification. Identification of isolates were done according to Kreig et al (1984) based on staining and Bio-chemical tests (Catalase, Oxidase, Indole, Methyl Red, VP test, Citrate utilization, Nitrate reduction, H2S production in TSI, Gelatin liquification and Urease).
Serotyping: The isolates were sent to National
Antibiogram: Antibiotic sensitivity test was performed according to the procedure of Bauer et al (1966) utilizing Mueller Hinton Agar plates (HIMEDIA) by placing 20 mm antibiotic discs. The following antibiotic discs were applied: Ciprofloxacin (Cf/ 10µg), Chloramphenicol (C/ 10 µg), Enrofloxacin (Ex/ 30 µg), Pefloxacin (Pf/ 5 µg), Norfloxacin (Nx/ 10 µg), Ampicillin (A/ 25 µg), Neomycin (N/ 30 µg), Gentamycin (G/ 10 µg), Apramycin (Ap/ 15 µg), Kanamycin (K/ 30 µg), Cephalaxin (Cp/ 30 µg), Tetracycline (T/ 10 µg), Cotrimoxazole (Co/ 25 µg), Nitrofurazone (Nr/ 100 µg), Chlortetracycline (Cte/ 30 µg), Erythromycin (E/ 15 µg), Streptomycin (S/ 10 µg), Colistin (Cl/ 10 µg), Oxytetracycline (OT/ 10 µg) and Lincomycin (L/ 5 µg)
Results and Discussion
In the present investigation,
In the present study, 65
The sensitivity and resistance pattern of these isolates for various antibiotics are presented in Table. It was observed that none of the antibiotics used were found to be cent percent effective. In this study, multiple antibiotic sensitivity and resistance pattern was observed in all of the examined strains similar to the findings of previous studies (Guerra
In conclusion, the findings clearly demonstrates multiple antimicrobial resistant