Antimycotic and Antibacterial activities of Gynandropsis pentaphylla DC extracts and its Phytochemical Studies
J Borgio, P Thorat, A Lonkar
antibacterial assay, antimycotic assay, phytochemical study
J Borgio, P Thorat, A Lonkar. Antimycotic and Antibacterial activities of Gynandropsis pentaphylla DC extracts and its Phytochemical Studies. The Internet Journal of Microbiology. 2007 Volume 5 Number 2.
The cat's whiskers 11 , 5 (
Kurhur has been used for several years in Indian traditional medical practices.
Materials And Methods
Collection of plant and preparation of extract
Qualitative phytochemical evaluation was carried out to test the presence of alkaloids, flavones, sugar, phenolic group, saponin, amino acid and essential oil in the extracts samples using modified method of Brindha
Pure culture of two human pathogenic bacteria such as one gram negative strain,
Luria agar (LA) and Luria broth (LB) and potato dextrose agar (PDA) and potato dextrose broth (PDB) (HiMedia, Mumbai, India) were used for the bacterial and fungal bio assay respectively.
Cloroamphenicol (10mg/ml) and fluconozole (10mg/ml) (HiMedia, Mumbai, India) were used as standard antibacterial and antifungal agent respectively. They were included in the current study as standard reference drugs.
Determination of antimicrobial activity
Sterile cottons swap was taken and dipped in 24 hours old
The zone of inhibition in extract and the standard antimicrobial agents were used to calculate the activity index.
The zone of inhibition was subjected to cluster analysis to reveals the relative ness of the activity among the human pathogens using STATISTICA/w 5.0. software. A correlation analysis between the pathogens and also different extracts were performed using STATISTICA/w 5.0. software.
Results and Discussion
The phytochemical screening of
The results of the antimicrobial activity presented in table 2 shows that all extracts exhibited appreciable antimicrobial properties, inhibiting the growth of all microorganisms. While stem aqueous extracts not inhibit the growth of
Generally, proportion index of antimicrobial activities of
The relationship between the sensitivity pattern of different microbials against various extracts used for the antimicrobial assay had been studied using STATISTICA/w 5.0. software (table 3).
A high correlation coefficient (near 1 or -1) means a good relationship between two variables and its value around zero means no relationship between them at a significant level of p < 0.05. More precisely, it can be said that parameters showing r > 0.7 are considered to be strongly correlated whereas r between 0.5 and 0.7 shows moderate correlation. In the present study, the resultant matrix reveals strong positive correlation among different bacteria (table 3). The relationship between the antimicrobial activity pattern of different extracts against various microbials used for the assay had been studied. Highest correlation (0.99) was observed in between aqueous extract of root and acetone extract of seeds. Higher correlation (0.97) was observed in between acetone extract of leaves and roots; acetone extract of stems and standard antibiotic. 0.96 correlation was observed in between aqueous extracts of leaves and seed pods.
A data matrix of five organisms and 19 variables (different extracts with one aqueous and two solvents) were used for this cluster analysis, the results are presented in figure 2.
The microbes were separated into two main groups I and II based on their sensitivity: the bacteria,
Presence of alkaloids and reducing sugars in leaves and stems were reported by Ajaiyeoba 5 . In the present study also we observed the same results in stem and leaves. Apart from both leaves and stems we additionally observed the presence of alkaloids and reducing sugars in seeds, roots and seed pods extracts of
From the result of the antibacterial studies as shown in Table 2, all the extracts exhibited appreciable antibacterial properties, inhibiting the growth of all the bacteria and fungi. The same results were repotted by Ajaiyeoba 5 , but the zone of inhibition was comparatively less in methanol extracts of stems and leaves. However, in the present study we noted the highest activity (28.4±0.0) against
In the antimycotic assay,
Conclusively, all extracts have displayed antimicrobial activities from present studies. The leaves and seeds of cat's whiskers are used in indigenous medicine in many countries 11 . This has further confirmed the use of this plants in Indian ethnopharmacology for treatment of bronchitis, boils, earache, eye wash, disinfectant and nasal congestion, analgesic, headaches, epileptic fits, facilitate childbirth in pregnant women, stomach-ache, constipation, conjunctivitis, thread-worm infection, chest pains, arthritis, inflammation, neuralgia, rheumatism, localized pains, pus, anaemia, uterine complaints, malaria, pneumonia, head lice and reduce coughing 10 , 3 , 17 , 14 , 11 , 16 , 5 . There is need for the development of new antibiotics due to acquired resistance, more importantly from natural sources as this delays resistance.
The senior author JFB is grateful to the Management and Mr. Jagdish Nagdev, Director, GeneCity Laboratory Pvt. Ltd Pune for the laboratory facilities. Many thanks to Prof. Mrs. Gandhe, Modern Collage, Pune, for plant identification. PKT and ADL are thankful to Mrs. Damle, Department of Biochemistry, Garware College, Pune for the encouragement.
J. Francis Borgio Genecity Laboratories Pvt. Ltd. 128 / 1B, First Floor, Chhatrapati House, Paud Road, Kothrud, Pune – 411038, India. E.mail: email@example.com Mobile: 0996005686