Prevalence And Antibiotic Resistance Pattern Of Methicillin-Resistance Staphylococcus Aureus Among In-Patients At A Tertiary Health Facility In Ido-Ekiti, Nigeria
F O.D, O E.I.O, A A.T, O O.T
antibiotics, methicillin-resistant staphylococcus aureus mrsa, methicillin-sensitive staphylococcus aureus, multidrug resistance.
F O.D, O E.I.O, A A.T, O O.T. Prevalence And Antibiotic Resistance Pattern Of Methicillin-Resistance Staphylococcus Aureus Among In-Patients At A Tertiary Health Facility In Ido-Ekiti, Nigeria. The Internet Journal of Laboratory Medicine. 2009 Volume 4 Number 2.
Infections due to MRSA are of special concern since they are always associated with prolonged hospital stay (as a result of few therapeutic options) and increased cost of treatment;2 in a prospective case-control study, the median hospital stay sequel to hospital acquired Methicillin Sensitive
The emergence of these MRSA is a direct result of selective antibiotic pressure which once generated become extremely difficult to control and as a result the knowledge of prevalence of MRSA with their antibiotic susceptibility pattern in any environment is of paramount importance in determining the best treatment options for patients infected with this strain of organism. This study aim at determining the prevalence of MRSA infection among hospital in-patients at Federal Medical Centre (FMC), Ido-Ekiti, Ekiti State, a tertiary health facility in South-Western Nigeria, and to examine the resistance pattern of these strains to antibiotic commonly used to treat
Material And Methods
The study was carried out at FMC, Ido-Ekiti, Ekiti State, Nigeria, between January and December 2009. All
Antibiotic susceptibility testing incorporating the following antibiotics; cefoxitin (10 µg, REMEL), penicillin (10U, REMEL), ciprofloxacin (5µg, REMEL), gentamicin (10µg, REMEL), erythromycin (15µg, REMEL), clindamycin (2µg, REMEL), cephalexin (30µg, REMEL), trimetoprim/sulphametoxazole (1.25/23.75µg, REMEL), and vancomycin (30µg, REMEL) was done using the modified Kirby-Bauer disc diffusion technique; a sterile cotton wool swab stick (Evans, UK) was used to inoculate the entire surface of Mueller-Hinton agar (MHA) plate (Oxoid, UK) with the inoculum of
A total of 158 strains of
This study, like previous studies, had demonstrated that MRSA are more resistant to various group of antibiotics compared to MSSA. 17,22,23 It is not surprising that all the MRSA tested in this study were resistant to penicillin and cephalexin whereas only 72.4% and 48% of MSSA respectively were resistant to these antibiotics; the finding only substantiates the fact that resistance to methicillin predicts resistance to other beta-lactam drugs.1 All
The study had demonstrated a high prevalence rate of MRSA with high rate of resistance to commonly used anti-staphylococcal agents. A large proportion of these MRSA were found to be multi-drug resistant. These findings call for urgent attention whereby strict antibiotic policy should be enforced to curtail irrational use of antibiotics with its attendant evolution of resistant strains of