Immunochromatographic detection of Helicobacter pylori antibodies in paediatric patients in Asaba, Nigeria.
J Isibor, A Asiodu
antibodies, asaba, children, helicobacter pylori, nigeria
J Isibor, A Asiodu. Immunochromatographic detection of Helicobacter pylori antibodies in paediatric patients in Asaba, Nigeria.. The Internet Journal of Laboratory Medicine. 2008 Volume 3 Number 2.
The taxonomy of the genus
Predisposing factors such as overcrowding of people, poor sanitation and living conditions can be found in most developing countries.  have reported that close person to person contact in childhood is an important determinant of seroprevalence of
Several diagnostic methods such as the pulsed-field-gel- electrophoresis , PCR , protein profiling  etc, have been used. These methods, no doubt, require special technologies, high cost and trained manpower factors that put them out of the ready reach of routine laboratory use in most third world countries. While the combination of culture and histological examination of gastric biopsy specimens has been considered the “gold standard” for the diagnosis of
Currently, the majority of routine serodiagnostic tests for
Here we report the prevalence of
Subjects and Methods
The subjects comprised of 100 children (aged 1 year – 16 years), who visited both the Federal Medical Centre (FMC) and the General Hospital (GH), Asaba. The children were first registered at the out-patients department by their mothers before being referred to the paediatrician for treatment. 80 of the children were clinically suspected of having gastrointestinal complaints while the remaining 20 children were apparently healthy, but needed medical check-up.
The consent of the hospitals’s medical directors and the childrens,s parents were obtained, before obtaining blood from the patients. About 5ml of blood was aseptically collected from each child by venepuncture and put into EDTA sample container; plasma samples were extracted, properly labeled and stored in a -200 C freezer at the end of each day.
The test device was carefully removed from the sterile aluminium pouch bag. A drop (50ml) of patients’ plasma was added into the sample well until it was completely absorbed; this was followed with a drop of chasing buffer solution. The preparation was then observed for 10-15 mins. A negative result was indicated when only one pink band showed up, thus indicating the absence of
A total of 100 children were screened for
The results of the 50 subjects screened at the GH are shown in Table 2. Out of the 40 subjects screened 14 (35%) tested positive for the antibodies while 26 (65%) were seronegative. 4 (40%) of the 10 control subjects were seropositive while 6 (60%) of them were seronegative for
In the present study, infection with
Of the 40 children screened at the Federal Medical Centre (Table 1), 8 (20%) tested seropositive, while of those screened at the General Hospital, Asaba, 35% showed seropositivity for
The faecal-oral route of transmitting infection may play a leading role for children’s infection since children’s poor sanitary habits make them easily vulnerable. We observe that the control specimens collected from the Federal Medical Centre and General Hospital, Asaba, tested seropositive (20% and 40% respectively) to
In this study an overall high seroprevalence rate of 55% children with gastrointestinal complaints has been recorded. High prevalence rates have also been recorded by other workers from other locations. In a survey of 268 subjects in Maiduguri, Nigeria, 85% of the people screened had lgG antibodies to
Overcrowding and poor sanitary conditions in most of the cities in developing countries often pave way for gastrointestinal pathogens to affect people living in these regions. Although there is yet no epidemic of