emm, pcr, streptococcus pyogenes
V Wiwanitkit. Analysis Of PCR Products From Using Emm Primers For Different Streptococcus Pyogenes Strains. The Internet Journal of Infectious Diseases. 2004 Volume 4 Number 2.
Materials and Methods
Emm primers and studied S. pyogenes strain
The emm primers used in this study are quoted from those developed by Pimtanothai
PCR amplification testing and sequencing of the PCR product
The PCR amplification was performed using the standard protocols proposed by Pimtanothai
According to the PCR amplification, the electronic analysis of the PCR product for each
In choosing the appropriate antimicrobial therapy, one must take into account the resistance profile of the target pathogen, the agent's antibacterial profile and the intrinsic activity against the target pathogen . Cunningham noted that emm gene sequencing had changed serotyping, and new virulence genes and new virulence regulatory networks have been defined . Cunningham also noted that the emm gene superfamily had expanded to include antiphagocytic molecules and immunoglobulin-binding proteins with common structural features .
During recent years, various new techniques have been adapted for the diagnosis of