Motility Of Intestinal Fluke, Echinostoma Spp, Metacercariae In Fish Dishes Prepared By Different Uncooked Methods
V Wiwanitkit. Motility Of Intestinal Fluke, Echinostoma Spp, Metacercariae In Fish Dishes Prepared By Different Uncooked Methods. The Internet Journal of Infectious Diseases. 2004 Volume 4 Number 1.
Fish-borne trematode is a worldwide problem. In addition to the major liver flukes, several types of intestinal flukes also use minnows as the intermediate host. Traditional ingestion of undercooked and uncooked fish preparations is a major factor in pathogen acquisition. Several freshly killed freshwater fish,
Fish-borne trematode is a worldwide problem, with the number of people infected by liver flukes alone estimated at 21 million. In addition to the diseases caused by major liver flukes,
Materials and Methods
Preparation of Uncooked Fish Dishes
The protocol of this study is the same as the previous reports (5, 6). Several freshly killed freshwater fish,
Motility Study of the Metacercariae
The motility of the metacercariae in each of these dishes was estimated under the stereoscopic microscope. Since non motile parasites may still be viable, the researchers excysted the immature fluke to study their motility. Motility was estimated as the level of activity or degeneration of the metacercariae at the start of the experiment and then every 30 minutes until all the parasites appeared degenerate (Table 1).
All metacercariae were active and motile at the start of observation. Degeneration was observed in all dishes after 4 hours, except for the dishes that were frozen or refrigerated, in which degeneration was observed after 10 hours.
Fluke infection is an important infectious disease in Thailand. Health education promoting cooked fish consumption for preventing further infection is one of the the main strategy for fluke control in Thailand(7). A recent survey indicated that ingestion of uncooked fish dishes was a risk factor for fluke infection.(8) An important factor in transmission of fluke infection via eating fish dishes is viability of the metacercariae possibly living in the fish.
Not only the liver fluke diseases but also the intestinal fluke diseases are endemic in Thailand. Here, we focus our study on the
Like the previous studies of the
The author has to thank Associated Professor Suwanne Nithiuthai, Department of Parasitology, Faculty of Veterinarian Sciences, Chulalongkorn University for her help in this study.