R Anantha Rajan, S Suganthi, A Sabitha, G Prakash Vincent
carbohydrate, haemagglutination, lectin, turbo
R Anantha Rajan, S Suganthi, A Sabitha, G Prakash Vincent. Isolation and Partial Characterisation of Lectin from Turbo sp.. The Internet Journal of Genomics and Proteomics. 2006 Volume 3 Number 1.
A calcium dependent lectin was purified from
The recognition of carbohydrate moieties in macromolecules by lectins has important applications in cell to cell interactions, signal transduction, cell growth and differentiation (Lis, & Sharon, 1991). Mollusc has variates of lectin with different biotechnological applications. Fenelson
Materials & Methods
500µl of the
In order to find out the optimum temperature for maximum activity and temperature tolerance the samples were incubated at different temperatures from 20°C to 90°C using water bath for 20 minutes and haemagglutination activity was determined. To find out the optimum pH, the extracts was dialyzed at pH 3 to 9 for 24 h and then in TBS for 24 h at pH 7.6. The samples were dialysed against TBS without calcium, with Ca 2+ and Mg and after 24 h haemagglutination assay was carried out. The sample was dialysed the TBS with 0.25M EDTA and haemagglutination activity was carried out and the same sample was dialysed with TBS after which the haemagglutination activity was estimated.
Results and Discussion
Muscle extract of
In the haemagglutination inhibition assay, the inhibitors Lactose, Glucose, Galactose, Fructose, Maltose, Mannitol, Mannose, N-acetyl neuraminic acid were used. None of them inhibited the agglutination. Muscle extract purified by
DEAE cellulose chromatography showed 4 peaks in 17, 20, 26 and 30 fractions as given in Fig. 1. Among these, the 7 th fraction showed titre value of 12 for rabbit erythrocytes. The extract of
The lectin from
Haemagglutination activity was inactivated in lower pH observed in higher pH of 7 and 8 as in fig 3. The haemagglutinating activity of extracts of mollusk,
The purified column fractions of
The authors are grateful to the authorities of Manonmanium Sundaranar University, Tirunelveli, for providing required facilities.
Address for communication
Dr. R. Anantha Rajan Lecturer, Centre for Marine Science and Technology, Rajakkamangalam-629 502, Tamilnadu, India. email@example.com