Staphylococcus Aureus As A Causative Agent Of Atopic Dermatitis/ Eczema Syndrome (ADES ) And Its Therapeutic Implications
I Al-saimaru, S Bakr, K Al-Hamdi
atopic dermatitis eczema syndrome, staphylococcus aureus
I Al-saimaru, S Bakr, K Al-Hamdi. Staphylococcus Aureus As A Causative Agent Of Atopic Dermatitis/ Eczema Syndrome (ADES ) And Its Therapeutic Implications. The Internet Journal of Dermatology. 2005 Volume 4 Number 2.
From 286 studied ADES cases, (94.4, 86.36) % from eczematous lesions and healthy areas gave positive bacterial cultures (P<0.05).
Antibiotic susceptibility of thirteenth antibiotics against
Atopic dermatitis/ eczema syndrome (ADES) is chronic relapsing, pruritic inflammation of the skin, affecting 10-20% of children and 1-3% adults, worldwide, with increasing prevalence in highly industrialized countries(1).
The aims of the present study are to determine presence / or occurrence ratio of
Material & Methods
A total of 286 patients (males & females) in various age groups were concluded in this study. The patients were suffered from atopic dermatitis / eczema syndrome (ADES) attending the out patients of department of dermatology of main hospitals in Basrah providence (out patients based study). ADES diagnosed under supervision of dermatologists based on criteria of Hanifin & Rajka, 1980(7), Spergel & Schneider, 1999(8), and Stanway, 2005(9). The study was carried out during a period from November 2003 to July 2005.
Skin swabs were collected from eczematous lesions and nearly healthy areas of ADES patients saturated by Brain Heart Infusion Broth (Oxoid), and transported immediately to the laboratory(10). And then cultured on primary isolation media: Blood Agar Base (Oxoid), MacConkey Agar, and Nutrient Agar (Himedia). And incubated at 37oc for 24-48 hrs aerobically. Samples that cultured on Chocolate Agar in addition to Nutrient Agar were incubated and CO2 in candle jar at the same temperature and period mentioned above(10).
API technique (bioMerieux, France) as a rapid identification system was used for identification of various bacterial isolates based on enclosed instruction of supplied company. Some specialized biochemical tests and grew on Mannitol Salt Agar and Staph 110 Agar (Himedia)-that used as a selective media-were done for confirmation the diagnosis of
Thirteen antibiotics (Himedia, India) common used with treatment of atopic dermatitis(6,13) were used to testing the antibiotics susceptibility of
Amoxycillin / Clavulanic acid (20/10mg) (AC), Bacitracin (10U)(B), Cephalothin (30mg)(Ch), Chloramphenicol (30mg)(C), Clindamcin (2mg)(cd), Co-trimoxazole (trimethoprim/sulphametaxazole) (1.25/23.75mg) (Co), Doxycyclin hydrochloride 30mg) (Do), Erythromycin (15mg)(E), Gentamicin (10mg)(G), Methicillin (5mg)(M), Rifampicin (5mg) (R) Tetracyclin (30mg)(T), and Vancomycin (30mg) (Va).
Chi-square test and ANOVA test were carried by using computer program SPSS ver. 11, and statistical similarities were carried by using Minitab program ver. 10.
Table (1) illustrates all bacterial types isolated from eczematous lesions and nearly healthy areas. The total number of positive cultures (270, 247) cases from 286 studied ADES cases in percentages (94.4, 86.36) % from eczematous and healthy areas respectively (P<0.05). In general, twenty bacterial types were isolated from both area separately and (959, 744) isolates with isolation ratio (3.35:1, 2.6:1) isolates: case were identified in each above area respectively.
Table (2) illustrate antibiotic, susceptibility pattern of
It has been found highly significant differences (P<0.01) between three modes of antibiotic susceptibility within the same antibiotics and between these modes of various antibiotics.
Table (3) determine the percentages of antibiotics resistance modes of
The statistical similarities between antibiotics affecting modes on
Our result revealed (94.4% and 86.36%) of positive cultures from total ADE cases (eczematous lesions and healthy areas) respectively.
Results of previous studies were evidenced our results interested on the importance of microbial factors on the pathogenesis of eczema and the therapeutical implications for the treatment of atopic dermatitis(6). Since then, our knowledge concerning the complex interaction between microbes and skin inflammation has improved dramatically and today the Gram-positive bacterium, Staph. aureus is recognized as an important triggering factors for the maintenance of skin inflammation and acute exacerbations of the genetically determined skin disease atopic dermatitis(14,15). Many studies showed a heavy colonization of AD with
Thirteenth antibiotics were tested against
IHSAN EDAN AL-SAIMARY; Assist. Prof. Dept. Microbiology, Coll. Medicine , Univ. Basrah, PO BOX 696 Ashar, Basrah, IRAQ E-mail: Ihsanalsaimary@yahoo.com