Ethanolic Extracts Of Vernonia Amygdalina And Ocimum Gratissimum Enhance Testicular Improvement In Diabetic Wistar Rats
O ASUQUO, A EDET, O MESEMBE, J ATANGHWO
diabetes, improvement, ocimum gratissimum, streptozotocin, testes, vernonia amygdalina
O ASUQUO, A EDET, O MESEMBE, J ATANGHWO. Ethanolic Extracts Of Vernonia Amygdalina And Ocimum Gratissimum Enhance Testicular Improvement In Diabetic Wistar Rats. The Internet Journal of Alternative Medicine. 2009 Volume 8 Number 2.
To study the effects of ethanolic extracts of
The utilization of herbal extract from plants found in fields and forests to treat various types of illnesses and diseases has increased over the years
Materials and Methods
Sections of testes of control rats showed normal testicular architecture with distinct seminiferous tubules composed of both Sertoli and spermatogenic cells. The spermatogenic cells were seen to be at the spermiogenic stage of differentiation. Interstitial cells of Leydig were also prominently interspersed between the seminiferous tubules (fig i). The diabetic control showed alteration and distortion of both germinal epithelium and seminiferous tubules. The peritubular tissue surrounding the seminiferous tubules and interstitial cells were altered. There was presence of vacuoles within the seminiferous tubules (fig ii). The testes of group C treated with VA had a more stable seminiferous tubules compared to group B. Vacuoles were observed, but were not numerous as those observe in the diabetic control. Few spermatogenic and Sertoli cells were found in the testes of these rats (fig iii). Group D rats’ testes treated with OG showed a better improvement; distinct seminiferous tubules were observed. Numerous boundary cells as well as Sertoli cells were found in their lining epithelium. Blood vessels were seen in the connective tissue of the seminiferous tubules (fig iv). The testes of group E rats treated with combination of VA and OG showed a significant improvement of testicular arrangement. There was increase in Leydig cells; well defined seminiferous tubules were seen. Lumen of seminiferous tubules was filled with active spermatids.
Fig i Photomicrograph of testis of control group A rats showing normal testicular architecture. Spc- spermatogenic cells, Sp- spermatids, St- Sertoli cells, Lc- Leydig cells.
Fig ii Photomicrograph of testis of group B diabetic-induced rats showing testicular damage. Dsc-degenerating spermatogenic cells, Bst-broken sperm tails, LLc- lost Leydig cells.
Fig iii Photomicrograph of testis of group C diabetic rats treated with
Fig iv Photomicrograph of testis of group D diabetic rats treated with
Fig v Photomicrograph of testis of group E diabetic rats treated with combination of
The incidence of diabetes among men attending fertility clinics and among men who are subfertile is high25. Since antiquity, diabetes has been treated with herbs; recent scientific investigations have confirmed the efficacy of these herbal preparations which are remarkably cheap and non-toxic26. The hypoglycemic effect of