V Rajamanickam, A Rajasekaran, S quine, M Jesupillai, R Sabitha
alangium salvifolium, diuretic, plant extract
V Rajamanickam, A Rajasekaran, S quine, M Jesupillai, R Sabitha. Diuretic activity of alangium salvifolium sub.sp.hexapetalum.. The Internet Journal of Alternative Medicine. 2008 Volume 8 Number 1.
Benzene and ethyl acetate extracts of
Materials And Methods
Plant collection and authentication
The plant material was collected in the Tirunelveli district, Tamilnadu, India. It was authenticated by Dr.V.Chelladurai, Govt. Research officer, Botany C.C.R.A.S. Govt. of India, (Retired), Tirunelveli. A voucher specimen has been kept in our laboratory (As) for future reference
Preparation of extract
The root bark of
Albino rats of both sex (150-250g) were collected and housed under standard laboratory conditions. They were fed with standard rat feed and water
The method of Lipschitz
The student ..t.. value was employed for statistical analysis. All the values expressed are Mean ± S.E.M.
Result and discussion
The preliminary phytochemical analysis showed the presence of flavonoids, alkaloids and steroids in both benzene and ethyl acetate extracts. All these extracts at 250 mg/kg showed increase in urine volume and also the concentration of Na+, K+ and Cl- in urine [Table 1]. From the present study, we concluded that the diuretic activity of Alangium salvifolium may be due to the presence of flavonoids in both the extracts.
Mean ± S.E.M, n = 6, Student ‘‘t’‘ test, *