A Chowdhury, M Ashraful Alam, M Rahman, M Rashid
antimicrobial, antioxidant, brine shrimp lethality, daucosterol, malvaceae, sida rhomboidea
A Chowdhury, M Ashraful Alam, M Rahman, M Rashid. Phytochemical and Biological Investigations of Sida Rhomboidea Linn.. The Internet Journal of Alternative Medicine. 2008 Volume 7 Number 2.
Materials and Methods
General experimental procedure
The 1 H NMR spectra was recorded using a Bruker AMX-400 (400-MHz) instrument. For NMR studies deuterated chloroform was used and the values for 1 H spectrum was referenced to the residual nondeuterated solvent signal.
Extraction and isolation
The powdered stems (600 g) of
The antimicrobial activity of the extractives was determined by the disc diffusion method 6 . The samples were dissolved separately in chloroform or methanol and applied to sterile filter paper discs at a concentration of 400 µg/disc. Kanamycin disc (30 µg/disc) was used as standard.
For cytotoxicity assay DMSO solutions of the plant extracts were applied against
The free radical scavenging activity of the extractives on the stable radical 1, 1-dipheny1-2 picry1hydrazy1 (DPPH) were estimated by the method of Brand-Willimams 8 . Here 2.0 ml of a methanol solution of the samples at different concentration were mixed with 3.0 ml of DPPH methanol solution (20µg/ml). The antioxidant potential was assayed from the bleaching of purple colored methanol solution of DPPH radical by the plant extract as compared to that produced by the standard antioxidant agents of
Results and Discussion
Compound 1 was isolated from the
In the antimicrobial screening, the extractives as
CT: carbon tetrachloride soluble fraction of the methanolic extract; DCM: dichloromethane soluble fraction of the methanolic extract; KAN: standard kanamycin disc (30µg/disc).
Following the procedure of Meyer 7 , the lethality
¬¬¬¬VS: vincristine sulphate (std); HX:
The antioxidant activity of various fractions of the methanolic extract was also determined. Table-3 shows antioxidant activity of the test samples. Here,
The results of antimicrobial, cytotoxic and antioxidant activities support the folk uses of
The authors wish to thank Centre for Biomedical Research, University of Dhaka, Dhaka-1000, Bangladesh for providing some laboratory facilities for carrying out the research.