M Jesupillai, M Palanivelu
anthelmintic activity, leaves, pheritima posthuma
M Jesupillai, M Palanivelu. Anthelmintic Activity Of Leaves Of Erythrina Indica Lam. The Internet Journal of Alternative Medicine. 2008 Volume 7 Number 1.
Ethanol, chloroform and ethyl acetate extracts of leaves of
Materials And Methods
The leaves were collected from Madurai District, Tamilnadu, India and authenticated by Dr. Stephen, Dept. of Botany, The American College, Madurai, Tamilnadu, India. A voucher specimen (EI 1) have been kept in our laboratory for future reference.
Preparation of the extract
The coarsely powdered leave materials were extracted exhaustively with Ethanol (95%), Chloroform and Ethyl acetate by using Soxhlet apparatus3. These extracts were concentrated under reduced pressure and preserved in desiccators until further use.
The method described by Dash et al45 was employed for evaluating anthelmintic activity.
Fifty milliliter of test solution containing 50 and 100 mg /ml of test extracts (Ethanol, Chloroform and Ethyl acetate extract of leaves of
The Mean time of paralysis and death was recorded in minutes. The paralysis time was recorded when no movement of any sort could be observed except when the worms were shaken vigorously. Time for death of worms were recorded when worms were neither moved while shaken vigorously nor when dipped in warm water (50°C).
Mean ± S.E.M, Student ‘‘t’‘ test, *P< 0.001 (Compared to standard) was considered significant.
All the values were represented as Mean ± S.E.M using student ‘‘t’‘ test. P< 0.001 (Compared to standard) was considered significant.
Result And Discussion
Ethanol, chloroform and ethyl acetate extracts of leaves of EI showed significant anthelmintic activity (table.1) at the concentration 50 mg/ml and 100mg/ml against Pheritima poshthuma. Activity was found to be increased with dose (shortest time of paralysis and death was observed at 100 mg/ml) and the activity was comparable to the well known anthelmintic agent Piperazine citrate.
Indian earth worms resembles intestinal round worm parasite of human beings.678 Anthelmintic activity of Piperazine citrate mediated through hyper polarization that leads to muscle relaxation and flaccid paralysis.9 Tannins produced anthelmintic activity by binding to free protein in the gastrointestinal tract of the host animal10 or glycoprotein on the cuticle of the parasite.11Phenolic compounds (tannins are poly phenolic compounds12) by uncoupling oxidative phosphorylation hinder the energy production in helminth parasites.13 Phytochemical analysis of leaves of
M.Jesupillai, S/O K.Muthuchellam, Kakkoor-623711, Mudukulathur (tk), Ramanathapuram (dt), Tamilnadu, India. E-mail: Javaharpillai@rediffmail.com