S Mahibalan, S Jasemine, M Jesupillai
anti inflammatory, paw edema, rats
S Mahibalan, S Jasemine, M Jesupillai. Anti inflammatory effect of methanolic extract of Hedyotis Umbellata Linn. The Internet Journal of Alternative Medicine. 2008 Volume 7 Number 1.
Materials And Methods
The plant material was collected in the Nellai district, Tamilnadu, India. It was authenticated by Dr. Stephen, Department of Botany, The American College, Madurai. A voucher specimen has been kept in our laboratory (HU1) for future reference.
Preparation of extract
The aerial parts of the
Anti inflammatory activity
The method of Winter CA et al  was employed for evaluating Anti inflammatory activity. Albino rats (Wister strain) of both sex (120-160gm) were collected from the animal house of our institute and provided with standard pellet with free access to water
Twenty four albino rats were divided in to four groups of six each. The group I animals received 10% aqueous tween 80, group II animals received Diclofenac (50 mg / kg), groups III and IV animals received Methanol extracts (Suspended in 10% aqueous tween 80) of
The statistical analysis were carried out by student ‘t’ test, P 0.001 was considered significant. All the values are reported as Mean±SEM.
Results And Discussions
MEHU at 250 mg/kg and 500 mg/kg significantly (P<0.05) reduce carrageenan induced paw edema. The percentage edema inhibition was found to be 52.27 % and 60.07 % for MEHU (250 mg/kg and 500 mg/kg respectively) and 71.92 % for Diclofenac sodium. Carrageenan induced paw edema is a acute inflammation model. It is believed that carrageenan induce paw edema by biphasic mechanism. The first phase is attributed to the release of vaso active amines such as histamine and serotonin.  and the second phase is continued by the prostaglandins (PG), SRS-A (Leucotrienes) and acidic lipids . From the result we conclude that Hedyotis umbellata possess anti inflammatory activity. The effect may be due to the inhibition of formation of several inflammatory mediators . Further study required to isolate active constituents responsible for the activity.
M.Jesupillai Dept. of Pharmaceutical Chemistry, Arulmigu kalasalingam College of Pharmacy, Krishnankoil, Pin code: 626 190. Virudunagar (Dt),Tamilnadu, India. E-mail: email@example.com