Insilico Analysis Of Cranberry Proanthocyanidin As An Inhibitor For Modelled Afimbrial Adhesin Virulence Protein Of Uropathogenic Escherichia Coli
M Abhilash, H Makari, H Ravikumar Patil
autodock, fimbrial adhesion, modeller 8v2, uropathogenic
M Abhilash, H Makari, H Ravikumar Patil. Insilico Analysis Of Cranberry Proanthocyanidin As An Inhibitor For Modelled Afimbrial Adhesin Virulence Protein Of Uropathogenic Escherichia Coli . The Internet Journal of Medical Informatics. 2007 Volume 4 Number 1.
Fimbrial adhesion is a Virulence Determinant which is classified under Adhesins category of virulence factor of uropathogenic
Among various organisms which are known to cause Urinary tract infections, Escherichia coli is the most predominant pathogen being isolated in 70-90% of cases. It has been accepted that UTI caused by
Different virulence factors of E.coli which are thought to have a role in the pathogenesis of Urinary Tract Infections are O Antigens, K Antigens, Hemolysins, Serum resistance, Adhesins, Capacity to produce mannose sensitivity and resistant haemagglutination. fimbrial adhesion is a Virulence Determinant which is classified under Adhesins category of virulence factor.
The term adhesin has been described as a microbial surface component that mediates specific attachment to eukaryotic cell membrane and encompasses well known fimbriae as well as other poorly characterized and undefined structures. Adherence is facilitated by E. coli Fimbriae which are proteinaceous fibers on the bacterial cell wall. Fim-briae produce adhesins that bind to specic carbo-hydrate receptors present on uroepithelial cells (Beachey, 1981).
Many bacteria are known to be adhesive, attaching to and living in close association with various surfaces in their natural habitat. The ability of many pathogenic bacteria to adhere to specific host tissues is a factor of primary importance in diseases such as bacterial diarrhea, gonorrhea and Urinary Tract Infections. This specific adherence plays two important roles
It allows the bacteria to resist and circumvent the flushing and cleansing mechanisms that protect many epithelial surfaces in higher animals.
It determines the site of microbial infection by facilitating specific surface interaction between the bacteria and host epithelium.
Many studies (Fowler et al 1977, Kallenius et.al 1978) have shown that bacterial adherence is an essential virulence factor in the pathogenesis of community acquired Urinary Tract Infections. Duguid et al (1966) studied fimbriae of
Cranberry juice acts by preventing adhesion which presumably helps urinary flushing of the causative bacteria, preventing their colonization of the urinary tract. This effect is achieved by inhibiting the infecting bacteria,
Action of Cranberry proanthocyanidins:
Alter E. coli's cell membranes
Prevent the bacteria from making contact with cells or attaching to them even if they somehow manage to get close enough
Change the shape of E.coli from rods to spheres
Disrupt bacterial communication
Materials And Methods
Protein with swiss-prot primary accession number P08180 , of length 161 amino acids was selected for modelling of Afimbrial adhesin AFA-I.
Modelling of target protein was carried out using
Validation Of Modelled Protein
Validation of modelled structure was carried out using Structure Analysis and Validation Server. Structure Analysis and Validation Server greatly simplifies computational analysis of the molecular structure and sequence of proteins. The stereochemical validation of model structures of proteins is an important part of the comparative molecular modeling process.
Active sites present in the protein were identified using
Docking analysis was carried out using AutoDock 4. Analysis of Epicatechin a
Results And Discussion
Validation of modeled structures obtained from Swiss-PdbViewer and MODELLER 8 V2 was carried out using Structure Analysis and Validation Server. The structure modelled using Modeller 8V2 and Swiss-PdbViewer had over all quality factors of 100% and 77.14 % respectively.
Modelled structure obtained from Modeller 8V2 was used as target protein for further analysis. Prediction of active sites was carried out using Q site finder. It was found that ALA 21, SER 107, MET 108, TYR 122 TRP 159 are potential active sites among several others.
Docking analysis of Epicatechin a Proanthocyanidin from cranberry as an inhibitor against modelled fimbrial adhesion was carried out using AUTODOCK 4. Best Binding energy was found to be -6.47 at the 6 th run. The details of results obtained from AUTODOCK 4 has been mentioned below.Interaction of Epicatechin with modelled fimbrial adhesion protein was analyzed using AUTODOCK 4 as mentioned in figures 2 and 3.
Some of the commonly used antibiotics to treat urinary tract infections caused by Uropathogenic
Since its reported that Uropathogenic E Coli have developed antibiotic resistance(Gupta et al 2002), Epicatechin Proanthocyanidin from cranberry with many evidences of its activity against virulence proteins of Uropathogenic
We thank Dr K.N Somasekharan, Dean, School of Chemical & Biotechnology, SASTRA UNIVERSITY, Mr Shivkumar , Mr Balamurugan ,SASTRA UNIVERSITY, Thanjavore, Tamilnadu, INDIA for their support.