anti bacteria activity, tea bag
T Mbata. Preliminary Studies Of The Antibacterial Activities Of Processed Kenyan And Nigerian Tea. The Internet Journal of Microbiology. 2005 Volume 2 Number 2.
The antibacterial activities of extracts in Kenyan and Nigerian tea bags were tested for activity against six organisms,
Tea is an infusion of flavorful leaves that has been consumed for centuries as a beverage and is valued for its medicinal properties. The phytochemcial screening of tea revealed the presence of alkaloids, saponins, tannins, catechin and polyphenols (Sofowara 1984, Opara 1992). Toda
Recent reports however indicate the tea's antibacterial and bactericidal properties on various bacteria strains isolated for patients with infected root canels (Horiba
Prompted by these reports, the study is aimed at investigating the antibacterial activities of extract in Kenyan and Nigerian tea bags on selected microorganisms.
Materials And Methods
Collection of sample
Two types of tea were used for this study. The Kenyan tea bought from Kenya industrial Research and Development Institute and the Nigerian Lipton tea produced in the mamblia Plateau Jos. Both tea were purchased in packets of 2g x 100bags.
The tea bags were aseptically and open with a sterile scissor and the extracts were prepared by the method as described by Toda et al . Tea extracts were suspended in cold phosphate – buffer saline (PBs) at 202, 10% and 5% W/V concentrations respectively. The suspension was held ay room temperature for 3 hours and then centrifuged at 15000 rev/min for 10 minutes.
Antibacterial Susceptibility Test: Agar-gel diffusion
The surface of sterile Mueller-Hinton agar plates was inoculated with 0.2ml of a 24h broth culture (106 cfu/ml of test organisms and evenly spread using bent sterile glass rod. Three wells of 6.0mm in diameter were aseptically punched on each agar plate using a sterile cork bore. Fixed volume 0.1ml of the tea extract was carefully placed in each well. The plates were then covered and incubated at 370C for 24h. The zone of inhibition in each well was obtained by measuring the underside of the plate in two planes with a ruler calibrated in millimeters. The control was placed with 0.1ml of the extracting solvent and incubated.
The effect of 5,10, and 20% concentrations of the Kenyan and Nigerian lipton tea extracts on selected bacteria are presented in Table 1. it can be seen that at all three concentrations the Nigerian lipton tea extract showed inhibitory effects on
The antibacterial effect of foreign brands of tea against
It was also observed that the antibacterial activity of tea extract was not demonstrated in all types of tea or in tea grown in all geographical locations for examples 20% extract of the Nigeria Lipton tea showed little or no effect on
This study has showed that the extracts in the Kenya and Nigerian tea bags have antibacterial properties against a number of bacteria. The potential utilization of these properties especially in relieving stomach cramp and weight loss is the subject of another study.