How To Elicit The Genotoxic Effect Of Aqueous Extract Of Ecballium elaterium Using Micronucleus Assay And DNA Single Strand Break Techniques
I Muhammad Said Shabbar
I Muhammad Said Shabbar. How To Elicit The Genotoxic Effect Of Aqueous Extract Of Ecballium elaterium Using Micronucleus Assay And DNA Single Strand Break Techniques. The Internet Journal of Health. 2006 Volume 6 Number 2.
Medicinal green plants are generally containing mutagenic and carcinogenic substances, but there is little information about the biological activities of herbal medicine.[
Dioscorides applied it to the medicine of which we are treating. [
A recent study conducted was designed to investigate the anti-inflammatory effect of aqueous extract of
A study has been made taking a series of 13 patients who were exposed to the juice of
Cucurbitacin E (CuE) extracted from
was about 25% lower than that of PHA, but a combination of CuE and PHA had an additive effect producing a greater response than that induced by the two substances used separately. Agarose gel electrophoresis for DNA fragmentation failed to show any significant apoptotic activity in the cells after 48 hrs exposure to CuE 
The immunomodulatory effect of CuE, was tested in peripheral human lymphocytes. These lymphocytes were co-cultured with cancer cells and an interesting lymphocyte-mediated cytotoxicity was observed.  A Spectral Assignments and Reference Data has been done by 2D NMR-derived 1 H and 13 C NMR signal assignments of six structurally closely related cucurbitacin derivatives. The investigated aglyca CuE and I were isolated from
The effect of cucurbitacin of
The micronucleus assay is a cytogenetic test commonly used in various biological systems for monitoring environmental genotoxicity, both
Detection of single strand breaks
DNA strand breaks can be categorized as either single-strand break (SSB) or double-strand break (DSB). SSB normally represent reparable lesions, because the opposite strand holds both ends close together. In contrast, however, double strand breaks are usually considered to be lethal, because they are not easy repairable. Anyhow, detection of DNA strand breaks, either directly induced, or transiently induced during repair processes can be considered as a helpful tool to test the genotoxic properties of chemicals and particles .