A Nwaziri, G Ezeifeka, E Amadi
impregnation., infected, infertility, offspring, pregnancy, rats
A Nwaziri, G Ezeifeka, E Amadi. The Effect Of Gardnerella Vaginalis On Infertility And Pregnancy Of Albino Rats. The Internet Journal of Gynecology and Obstetrics. 2009 Volume 12 Number 2.
The effect of
The normal vagina is colonizes predominantly by Lactobacillus which are believed to afford protection against microbial pathogens. De-population of lactobacilli from normal vaginal flora and over-growth of
The involvement of microorganisms in infertility and pregnancy associated problems has long been widely described 6,7,8,9,10,11,12. Although some workers have reported isolation of GV from semen samples13, its actual involvement in infertility and or pregnancy-related complications has not yet been very explicitly highlighted. This study attempts to determine the possible effect of GV on infertility and pregnancy complications in albino rats.
Materials And Methods
Selection of albino rats
Forty male albino rats of about 3 months old weighing between 120-150g were randomly selected from the animal house of University of Nigeria Teaching Hospital (UNTH), Enugu. Similarly, another forty females were selected. Also, fifteen pregnant rats were randomly selected from the same animal house.
Infection and Mating of albino rats
Twenty female and ten male albino rats were inoculated with 0.02ml of
The rats were monitored on weekly basis until delivery. The number of rats impregnated, number of offsprings produced and survival rates of the offsprings after seven days were determined and recorded.
All the rat groups were reared under the same condition with commercially available rat feed and watered
Determination of the effect of on gravid rats
Ten pregnant rats were inoculated intra-vaginally with 0.02ml of GV containing 105 CFU/mL suspension. The pregnant rats were fed and watered
All the pregnant rats were observed until delivery to determine the number of healthy rats delivered, their weight and possible pregnancy complications.
Results And Discussion
The result of the study showed that the mating of matured female rats infected with 0.02ml of
The drastic reduction in the number of impregnations from 100% in the control group to 60-80% in the infected rats tend to suggest that GV infection may have some association with the infertility of the studied rats. Previous studies have associated microbial infections with cases of infertility. A significantly high incidence of genital infection was reported among male patients with idiopathic infertility than their fertile controls 8. Also, two separate researches reported the isolation of
It is apparent from the result of this work that infection with GV could pose untoward effect on the formation of fetus and survival of the new borne rats. Although the definite effect of this organism in human subject is yet to be elaborated, it is very likely to pose serious challenges, going by the presentation in the albino rats studied. This obviously demands further clarification to determine the probable role of this organism in infertility in humans.
This study also revealed that 20% of the GV infected gravid rats had abortion and at least two offsprings delivered per mother were feeble/underweight (Table 2). This observation supports earlier findings that microbial infections are incriminated in causing sporadic spontaneous abortion7 . It further corroborates other previous reports in which GV has also been associated with preterm birth, preterm rupture of membrane, chorioamnionitis and amniotic infection14,15 .
Generally, it does appear that the probable role of GV infection in infertility and pregnancy may not be contrary to the established adverse effects of sexually transmitted infections(STI). This calls for special attention since hitherto, GV have not been among the targeted organisms suspected to be critical in infertility and pregnancy complications. This study therefore underscores the urgent need for further research on the role of GV in infertility and pregnancy in both experimental models and human subjects.