In Vitro Antioxidant Activity Of The Hexane And Methanolic Extracts Of Cordia Wallichii And Celastrus Paniculata
H Makari, N Haraprasad, P Ravikumar
antioxidant activity, dpph scavenging, hexane extract, reducing power
H Makari, N Haraprasad, P Ravikumar. In Vitro Antioxidant Activity Of The Hexane And Methanolic Extracts Of Cordia Wallichii And Celastrus Paniculata. The Internet Journal of Aesthetic and Antiaging Medicine. 2007 Volume 1 Number 1.
In-vitro antioxidant effects of the hexane and methanolic leaf extracts of
Antioxidants are radical scavengers which protect the human body against free radicals that may cause pathological conditions such as ischemia, anaemia, asthma, arthritis, inflammation, neuro-degenertion, Parkinson's diseases, mongolism, ageing process and perhaps dementias (Polterat, 1997) Flavonoids and flavones are widely distributed secondary metabolites with antioxidant and antiradical properties. (Nakayoma and Yamada, 1995).
Plants are potent biochemical factories and have been components of phytomedicine since times immemorial; man is able to obtain from them a wondrous assortment of industrial chemicals. Plants based natural constituents can be derived from any part of plant like bark, leaves, flowers, roots, fruits, seeds, etc (Gordon and David, 2001) i.e. any part of the plant may contain active components. The beneficial medicinal effects of plant materials typically result from the combinations of secondary products present in the plant. The medicinal actions of plants are unique to particular plant species or groups are consistent with this concept as the combination of secondary products in a particular plant is taxonomically distinct (Wink, 1999). Antioxidant-based dugs/formulations for the prevention and treatment of complex diseases like atherosclerosis, stroke, diabetes, Alzheimer's disease, and cancer have appeared during the last 3 decades (Devasagayam
This has attracted a great deal of research interest in natural antioxidants. Subsequently, a worldwide trend towards the use of natural phytochemicals present in berry crops, tea, herbs, oilseeds, beans, fruits, and vegetables has increased. Several herbs and spices have been reported to exhibit antioxidant activity, including rosemary, sage, thyme, nutmeg, turmeric, white pepper, chili pepper, ginger, and several Chinese medicinal plants extracts (Lee
Materials And Methods
Plant samples of the selected species viz.
Preparation of extracts
10 g of the dried powdered samples from plants
The antioxidant activity of Plant extracts were determined by different in-vitro methods such as, the DPPH free radical scavenging assay and reducing power methods. The different extracts were dissolved in methanol at the concentration of 2mg/ml. all the assays were carried out in triplicate and average value was considered.
(a) DPPH Radical scavenging activity:
DPPH scavenging activity of the plant extract was carried out according to the method of Koleva
% DPPH radical-scavenging = [(Absorbance of control – Absorbance of test
Sample) / (Absorbance of control)] ? 100Control was the DPPH solution without plant extract.
Purified sample 2mg/ml in Methanol of
(b) Reducing power
This was carried out as described previously (Yildrim
Increased absorbance of the reaction mixture indicates stronger reducing power. In this study, hexane and methanolic leaf extracts of
Result And Discussion
1. DPPH scavenging activity
The percentage of DPPH racial scavenging activity presented in Table 1(a).
The methanolic extract of
The % of DPPH racial scavenging activity of hexane extract of
The methanolic extract of
2. Reducing power
Different extracts of
The reducing power of
Against the backdrop of many known medicinal properties of these plants, results from the present work suggest that relatively low values of antioxidant and reducing power may not imply a low medicinal value. Emerging trends in antioxidant research point to the fact that low levels of phenolics (and other phytochemicals) and low value of antioxidant indices in plants do not translate to poor medicinal properties. The present investigation indicates that through
The authors are grateful to Sri G M Lingaraju, Secretary and Dr. B T Patil, Principal GMIT, Davangere Karnataka, INDIA, for their financial assistance and lab facilities. The authors wish to thank all the staff members of the Dept of Biotechnology of GMIT and SJCE Mysore, Karnataka.INDIA.
Makari H. K Department of Biotechnology, G M Institute of Technology Davangere-577006 Karnataka , INDIA . firstname.lastname@example.org, Contact number - 91 8192 233345, Fax-918192 233344 Cell: 91 99642 66444